Part:BBa_K1583113:Design

pAra + fusion protein BFP_Spycatcher_His

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 131
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 71
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The start condon of SpyCatcher and the stop codon of BFP needed to be removed to ensure that one complete protein was expressed.

Source

These part was synthesized as linear DNA and only restriction/ligation was done to insert this into the backbone.